Add comment Close comment form modal. The proteins are aligned at their helicase domains.
Volume 62. Download all figures. However, not all the enzymes discussed in this review have been characterized in detail in plants yet. RECQsim also contains an insertion of mainly acidic residues in its helicase domain.
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Interestingly, these are the FA genes for whose products a biochemical function could be shown. Google Scholar.
Meiotic recombination is affected as well, when some of these helicases are missing. Specialized endonucleases resolvases make symmetrical cuts at the junction points step X , thereby leading either to CO or NCO products depending on the orientation of the cut step XI.
Common cellular phenotypes are disturbances in the regulation of the cell cycle and apoptosis, spontaneous chromosome breaks and radial chromosomes, as well as sensitivity against interstrand cross-link-inducing genotoxins such as mitomycin C Neveling et al. The roles of proteases during developmental programmed cell death in plants. Holger Puchta. Klutstein et al.
Multifaceted role of the Saccharomyces cerevisiae Srs2 helicase in homologous recombination regulation. Os RECQ1 has been shown to be required for transcription from loci that are able to form cruciform structures, possibly pointing to a role in RNA silencing H.
Gene targeting, which is too rare an event to be useful in plant biotechnology at the moment, was reported to be enhanced by up to two orders of magnitude by expression of yeast Rad54 in Arabidopsis plants Shaked et al.
Following a DSB, induced either by a protein like Spo11 in meiosis or by accident through irradiation or a chemical agent in somatic cells, the ends are resected to produce single-stranded 3' overhangs. Higgins and F. First, there are genes that were bioinformatically identified as helicases as they show sequence similarity to domains of known helicase families, but they do not have any intrinsic ATPase or DNA-unwinding activity.
Isolation and characterization of the RAD54 gene from Arabidopsis thaliana.
This modification suppresses translesion synthesis and promotes PRR. In the undamaged lagging strand, overshoot synthesis will elongate the nascent daughter strand past the position of the lesion in the leading strand. Whether the latter two proteins indeed have a function in genome stability of chloroplast DNA awaits future mutant analysis.